But without any insert the fragmentation does not work and many reads still have this technical sequence. ![]() Actually the TSO should not be present in the final read after addition of Illumina adapters. In the second sample there are many cases with no insert and the TSO is followed by the poly A sequence. This is a simplified illustration from the 10x manual:Īs you can see the cDNA insert should be between the polyA capture probe and the TSO. The over-represented sequence is the template switching oligo (TSO) that is used for the synthesis of the second strand (the example is for the v2 chemistry but the principle is the same for v3 and later). C tubes and M tubes may be obtained through Miltenyi Biotech.I actually figured out what was going on some time ago: Single-cell suspensions or thorough homogenates are obtained using single-use, sterile C Tubes or M Tubes. All eight positions can be operated independently. It also allows for the creation of user-defined programs. The Miltenyi Biotec GentleMACS™ Octo Dissociator with Heaters is a benchtop instrument for the fully automated and standardized tissue dissociation or homogenization of up to eight samples.Įquipped with eight individual heating units and ready-to-use gentleMACS programs, it offers a fully-automated workflow for tissue dissociation. Miltenyi Biotec GentleMACS Octo Tissue Dissociator By appointment, researchers may utilize our GentleMACS instrument to develop fully automated protocols for dissociating their tissues of interest into cell suspensions ready for downstream applications. ![]() For further details and pricing, please contact us.Īs part of our mission to provide high quality single cell sequencing data, we offer walk-up access to our Miltenyi Biotech GentleMACS Octo tissue dissociator with heaters. We offer detailed analysis of your single cell data. We collaborate with the Botson University Microarray & Sequencing Core to provide you with the best sequencing for your library via Illumina NextSeq 5 platforms. ![]() ![]() 10X Chromium Single Cell solutions can capture between 500 – 10,000 cells from samples with cell concentrations of 100 – 2000 cells/uL. This enables novel discovery of gene expression dynamics and molecular profiling of individual cell types in an affordable and simple workflow. Specifically, The Chromium Single Cell 3’ Solution provides a comprehensive, scalable solution via high-throughput single cell characterization and gene expression profiling. Single Cell Services 10X Genomics Chromium™ Systemġ0x Genomics’ Chromium System is a unique system that partitions cells and prepares sequencing libraries in parallel such that all fragments produced within a partition share a common barcode. Uncover critical insight with our services. We offer multiple single cell library preparation options and next-generation Illumina sequencing via our collaboration with the BU Microarray & Sequencing Core. As a high-throughput core, we provide an array of services to get you from sample to high-quality single cell gene expression data and/or analysis.
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